Cctv news: Pay attention to the situation of valsartan in Zhejiang Huahai Pharmaceutical Company. Yesterday (July 29th), the spokesperson of National Medical Products Administration introduced the situation of trace N- nitrosodimethylamine (NDMA) impurities detected in valsartan raw materials of Zhejiang Huahai Pharmaceutical Co., Ltd.　

　　On July 6th, Zhejiang Huahai Pharmaceutical reported to National Medical Products Administration that a small amount of N- nitrosodimethylamine (NDMA) impurities were detected in the raw materials of valsartan for export, and voluntarily disclosed relevant information to the public according to relevant regulations and requirements.

　　Zhejiang Huahai Pharmaceutical Co., Ltd. immediately suspended the release and delivery of all valsartan raw materials in the domestic and international markets after detecting the impurity, and initiated the measures of voluntary recall. As of July 23, Zhejiang Huahai Pharmaceutical has completed the recall of all raw materials in China.

　　In 2012 and 2013, the current process of raw materials of this enterprise was approved by the European Drug Administration (EMA) and the US Food and Drug Administration (FDA) respectively.

　　Since July 6th, National Medical Products Administration has maintained close communication and contact with the European Drug Administration and the US Food and Drug Administration, paid close attention to the announcements and trends of risk assessment issued by international regulatory agencies, and organized experts to carry out risk assessment.

　　According to the toxicological data, the maximum daily intake limit of N- nitrosodimethylamine is 0.1μ G, which is equivalent to the provisional reference limit value of 0.3ppm by the European Drug Administration (calculated by taking 320mg valsartan daily).

　　According to the above-mentioned limit value, National Medical Products Administration conducted risk investigation on all the seven domestic manufacturers of valsartan raw materials (including Zhejiang Huahai Pharmaceutical). Except for the impurity of N- nitrosodimethylamine in valsartan raw materials of Zhejiang Huahai Pharmaceutical, the impurity detection value of N- nitrosodimethylamine in other domestic manufacturers of valsartan raw materials was lower than the limit value or was not detected.

　　There are 6 pharmaceutical manufacturers involved in the use of valsartan in Zhejiang Huahai Pharmaceutical:

　　The valsartan capsule (National Medicine Zhunzi H20103521) produced by Hunan Qianjin Xiangjiang Pharmaceutical Co., Ltd. has not yet left the factory.

　　The impurities of N- nitrosodimethylamine in the listed products of the other five manufacturers exceeded the limit, namely

　　Valsartan Hydrochlorothiazide Capsule of Chongqing Kangkeer Pharmaceutical Co., Ltd. (National Medicine Zhunzi H20080097)

　　Valsartan dispersible tablets of Hainan Huanglong Pharmaceutical Co., Ltd. (National Medicine Zhunzi H20050508)

　　Valsartan dispersible tablets of Harbin Sanlian Pharmaceutical Co., Ltd. (National Medicine Zhunzi H20061058)

　　Valsartan Hydrochlorothiazide Dispersible Tablets of Jiangsu Wangao Pharmaceutical Co., Ltd. (National Medicine Zhunzi H20090262)

　　Valsartan dispersible tablets of Shandong Yijian Pharmaceutical Co., Ltd. (National Medicine Zhunzi H20090319)

　　The above five preparation manufacturers have stopped using the raw materials of Valsartan of Huahai Pharmaceutical and recalled relevant drugs according to regulations.

　　National Medical Products Administration has asked all provincial food and drug supervision and management departments to urge relevant preparation manufacturers to take recall measures, and disclose relevant recall information on the websites of five manufacturers, including the contact numbers of the enterprises responsible for the recall.

　　In order to facilitate the public to know in time whether the Valsartan drugs being used are within the scope of recall, five manufacturers have cooperated with information technology companies and launched the code scanning inquiry function at 22: 00 on July 26, 2018. The product traceability code can be scanned through the mobile APP to realize instant inquiry.

　　National Medical Products Administration spokesman stressed that besides the above five preparation manufacturers, other valsartan preparation products are not included in the recall.

　　At present, European Union and other multinational drug regulatory agencies believe that N- nitrosodimethylamine belongs to Class 2A carcinogen (that is, there is sufficient evidence in animal experiments, but the evidence is limited), and it may be exposed to this substance in daily life (such as preserved food). Analysis of the drugs involved this time will not cause serious health risks to patients, but for safety reasons, risk control measures such as stopping sales and recalling should be taken.

　　On July 27th, FDA issued a notice that patients taking recalled valsartan should continue to take the current drugs until doctors or pharmacists provide alternative drugs or different treatment schemes.

　　A spokesman for National Medical Products Administration reminded that patients who are taking valsartan drugs must not stop taking them without authorization, because the risk of stopping taking them without authorization is more direct and serious for patients with hypertension. Whether to stop taking medicine or change medicine must be carried out under the guidance of a doctor. You can contact a doctor to replace other drugs containing valsartan that are not involved in the recall or choose other drugs for alternative treatment.

　　National Medical Products Administration requires the relevant provincial food and drug supervision and management departments to continue to follow the relevant provisions of the Measures for the Administration of Drug Recall, urge enterprises to do a good job in product recall and protect people’s drug safety.

1905 movie network news On November 20th, director Chen Kaige was invited to attend the film academic observation jointly organized by the Academic Affairs Office of Beijing Film Academy and the director department, and shared the film from scratch with the teachers and students present after the screening. More than 700 seats in the standard screening hall were packed, and the teachers and students in the school cherished the opportunity of face-to-face communication with director Chen Kaige.

　　　

In the post-screening exchange, Chen Kaige not only sincerely thanked the creative team of that year for "all their great contributions"; I also talked with you about the wonderful past that Leslie Cheung was Cheng Dieyi, and frankly said that "this experience is only this time"; He also expressed his concern and support for young creators, bluntly saying that "the film festival is not only a promised place for movies, but also an inspirational place representing youth and hope", and he hoped that the film festival could focus more on young creators.

　

Sincerely thank all the founders for sighing that Leslie Cheung has given us the only experience.

　　　

After the academic observation and screening of the film Farewell My Concubine, the film director Chen Kaige took the stage to share his feelings with all the teachers and students. Thanks to the hundreds of staff members who fought together in those years, especially Gu Changwei and Tao Jing, the alumni of the same session directed by Chen Kaige, "Photographers and sound recorders were very young at that time, but they created such magnificent images and powerful voices", as well as assistants and collaborators at that time, Zhang Jinzhan, Bai Yu, artist Zhanjia Yang and composer Zhao Jiping, "all made great contributions". ZhangFeng yi, one of the leading actors, is a leader in the performance department of Class 7 and Class 8 of Nortel. Director Chen Kaige highly praised his performance, saying that "his Duan Xiaolou is wonderful" and was moved by Mr. ZhangFeng yi’s dedication in filming.

　Director Chen Kaige also talked with you in more detail about the origin of filming, from being invited twice by producer Feng Hsu at Cannes International Film Festival to having various exchanges with original author Li Bihua. He not only gave Feng Hsu the first credit for Farewell My Concubine, but also praised Li Bihua as "the mother of Farewell My Concubine, and her novels laid an excellent foundation for the film" and "man of great talent, fantastic ideas". Li Bihua, as one of the screenwriters, also traveled from Hongkong to Beijing during the script creation stage to participate in the exchange and discussion between director Chen Kaige and another screenwriter, Reed. One of director Chen Kaige’s creative habits is to communicate with the screenwriter for a long time in the script stage, give a big outline for the characters, plot and story direction, and then discuss with the screenwriter, and finally realize it.

Especially in Farewell My Concubine, the time has been extended to 50 years, and the script structure is very complicated. In about half a year, director Chen Kaige and two screenwriters, Li Bihua and Reed, went through the process of affirmation, negation and reaffirmation. There is no routine, only the understanding and confirmation of the characters. After the first draft of the script was written, Reed took a pen and asked Chen Kaige to draw a circle in the satisfied scenes. If he was not satisfied, he would revise it again. After reading it, Director Chen Kaige only drew a few circles in the first draft, and then took everyone into a new round of discussion and revision. This manuscript is still precious. The script has been polished over and over again, and it is constantly enriched and perfected. Especially at the end of the script, Chen Kaige emphasized that the ending of the two people meeting in the bathroom in Hong Kong has no power. Only letting Cheng Dieyi follow the pace of Yuji’s fate is the only reasonable treatment that conforms to the character’s character logic.

　　

　In the actor’s part, Chen Kaige naturally talked about Leslie Cheung along the role of Cheng Dieyi, and what Chen Kaige always remembers is his first meeting with Leslie Cheung — —

　　　　

"The first time I met Leslie Cheung was when the first draft of the script was not finished. I have to tell him this story orally. Ms. Feng Hsu made arrangements for meeting in advance at the coffee shop of Mandarin Hotel. In the future, he jumped down from the same building. He is very gentle and quiet, and I speak in a hurry, for fear that we will have a language barrier. I speak Mandarin, and he is an actor who speaks Cantonese. I’m afraid what I said won’t impress him. I have also said on other occasions that he smokes a lot and his fingers are shaking all the time. In the process of speaking, I was skeptical and psychologically repulsive, so I asked myself, how do I know that he is the right person to play Cheng Dieyi? I don’t even know if he is a good actor. My story happened in China, and he is a Hong Kong person. Can he understand such characters and stories? And here I am, madly telling a story that may be rejected.

He never spoke, just listened, sometimes he looked up at me, and sometimes he didn’t look at me. But when I finished speaking, it suddenly dawned on me that he was Cheng Dieyi. Because I think he is like a person sitting in the bow of this story ship. After the ship moves, the lakes and mountains are changing all the time, and these light, shadow and water waves all reflect on his face. I don’t want to say that he is acting, but he is following Cheng Dieyi closely, approaching him, expressing him and loving him in a very subtle way. At this time, he stood up and shook hands with me and said,’ Thank you for telling me the story. I am Cheng Dieyi.’ This is a hair-raising moment. "This also made Chen Kaige identify with Leslie Cheung — — This is the only experience.

　

Looking back, I hope the film festival will pay attention to young people and create new possibilities.

　　

After the film Farewell My Concubine was born, it won the Palme d ‘Or Award at the 46th Cannes International Film Festival in 1993, and won the Golden Globe Award for Best Foreign Language Film in the United States and two nominations for Best Foreign Language Film and Best Photography at the Oscars the following year. It was rated as one of the 100 best films in the world by Time Magazine, which has produced great international influence.

　　　

From Farewell My Concubine, Chen Kaige, the director, fought in major film festivals with his works in his youth, and the film festivals did give him honor and support. In Chen Kaige’s view, a good film festival "is full of charm and becomes the hall that filmmakers all over the world yearn for because it is unknowable." "You don’t know what kind of movie form a new trend will run out, and the new possibilities that keep emerging surprise you." Therefore, the film festival "is not only the promised land of movies, but also an inspirational place representing youth and hope". Chen Kaige believes that film festivals and professional organizations such as China Film Directors Association should pay more attention to those "young people who are happy, confused, miserable and give up everything for movies" and let them stand on the podium.

　　　

Director Chen Kaige’s sharing touched the teachers and students of Nortel who were present. In the subsequent interactive session, Chen Kaige answered the questions raised by the students in the audience one by one, and his serious and professional attitude moved people.

Special feature of 1905 film network When the opera meets the film, since its release, it has been evaluated with the high score of Douban 8.2 by virtue of the audience’s good reputation.

As the first 4K panoramic Cantonese opera film, it was shortlisted for the best opera film in the Golden Rooster Award for Chinese films, and also won the Golden Coconut Award for the best technology in Hainan Island International Film Festival. Through film technology, traditional Chinese opera is given a new look, and behind this, it is also inseparable from the credit of editing technology.

The editor of this film is a national first-class editor. His previous representative works include Youth Fight, Deep Sea Sword and Men’s Gang. Sun Yiqun’s mother is also an editor. She is Yang Xingyuan, the former president of China Film Editing Society, and her representative editing works are various.

Can editing technology be inherited? In this issue of Behind the Scene, Yang Xingyuan and Sun Yiqun, the editors, lead us into china national film museum to reveal the history of film film editing, and experience the technological changes of film editing together from the film age to the digital age.

Behind the scenes: Film editor Yang Xingyuan reveals the tools of "film film editing"

Film clip: "Manual sewing machine"

I have a particularly cordial feeling. I can feel the temperature of all these devices. Entering china national film museum, Yang Xingyuan knows a lot about the film editing machines in the museum.

　

Facing a splicing machine full of historical colors, Yang Xingyuan introduced that Premier Zhou Enlai cared about film splicing and specially entrusted Tianjin No.2 Optical Instrument Factory to develop this splicing machine for them, also known as the four-way sound painting machine.

From the end of the 1960s to the end of the 1990s, this was the only tool for their editors, and the frequency was controlled in the frequency of 24 squares by hand. "My editing of Man to Middle Age, Zhou Enlai and Armageddon was cut out by such a machine."

The process of film editing is particularly complicated. After the film is finished, it should be washed in the studio, and then printed into samples with the negative, and the editor and the director and other producers will jointly review it.

　

When editing, the sound and picture of the sample should be synchronized with the negative, so the first step of editing is not to cut, but to arrange, and each of the specific scenes, shots and sound tapes in all the shooting materials should be completely recorded first, otherwise the material synchronized with the negative can not be found later.

　

"So it’s not easy to start editing. All this work has to be done before you can enter the creative stage." Yang Xingyuan said that it is more difficult at the stage of revision. For example, if there are ten films and one place is revised, these ten films will have to be hand-cranked from beginning to end on the film receiver. "After shaking it down, it is a lot of films, and then it is lifted one by one. At that time, the state positioned the editor as a light manual worker. "

Recalling the editing work of "Zhou Enlai", at that time, the version of mixed editing of picture and sound was completed, but the director had to modify the picture temporarily. "I was so angry that I wouldn’t change it for you, but he personally took a dozen films upstairs and asked me to change it." In order to make the film better, Yang Xingyuan said that even if the editing process was not easy at that time, it still had to be modified.

In the past, when editing technology was underdeveloped, a small machine supported the creation of many excellent classic films. Sun Yiqun recalled, "At that time, my mother stayed up late at night, so I slept next to her with a small quilt. I always wanted to shake this film, and my mother was particularly afraid that I would destroy it. She said that if you broke it, the consequences would be unimaginable."

Nowadays, computer technology is very simple to operate the effects of transition editing, such as fade-in, fade-out, cut-in and cut-out, overlapping, etc., but it is very troublesome in the film age. It is necessary for the editor to make specific marks on the film with a special pen, and then let the special effects producers use optical skills to make them on the film.

　

The complicated and exquisite technology of film film editors bears the heavy history of China films.

"For example, there are seven kinds of scenes to record a dialogue and an action now. I think the workload is very heavy. There are more than 200 shots as soon as I open the folder, but it is much more complicated than us to hear these things that the predecessors are editing. My mother sewed by hand at that time, and now it is equivalent to using an electric sewing machine. "

　

Hearing his mother’s introduction to film editing technology, Sun Yiqun felt a lot. "Now we have mastered new technologies and brought us convenience, while the older generation got through it like that and admired it."

Digital editing: White Snake’s secret recipe

The era of film editing has ended, and digital computer technology has been fully popularized. Nowadays, Sun Yiqun’s editing work is no longer just a simple operation technology, but also needs to integrate his own ideas at the creative level.

　

Talking about her latest film White Snake, Sun Yiqun said that in editing skills, she paid special attention to the balance between drama and film.

"There are many stylized things in traditional Chinese opera. Actors often communicate with the audience and the camera, which breaks the spatial illusion that the film has carefully established." Therefore, she will especially avoid editing the lens that highlights the "fourth wall" and choose more pictures of actors communicating with each other.

　

When the traditional Chinese opera performance is put on the movie screen, it is easy to have a magnifying effect. In addition, it is 4K quality, and any tiny expression of the actor can be clearly captured. When editing, Sun Yiqun will also avoid the scenes that will appear exaggerated on the big screen.

"The hand, eye, body and steps of an opera actor, his figure is a very important aspect of expression. If I edit excessively or even choose inappropriate scenery, it will destroy the good rhythm of the opera, so I still have to pick up its rhythm and grasp it well." How to find a moderate point is a difficult part of this editing.

　

Sun Yiqun is also worried that if the aria is too long, it will affect the audience’s desire to watch. Among them, there is a scene of "Broken Bridge and Residual Snow", in which lady white snake complains to Xu Xian after suffering, and the recorded aria is very long. She took the initiative to discuss the reduction with the director. "Otherwise, it would ruin lady white snake’s image and become a dissatisfied housewife." Finally, after coordination and careful cutting, it was cut for more than 30 minutes.

White Snake reinterprets classic stories and boldly tries new editing techniques of combining drama with film. Yang Xingyuan feels that today’s editors have a higher pursuit than the older generation. "They need to have the ability to master the lens and handle the structure, not the things we have learned. Those things have passed. They can put more energy into creation. "

Two generations of editing methods

Yang Xingyuan was originally an actor, but later transferred to Changying and changed to editing. "Having been an actor for more than ten or twenty years, I can still understand the performance and rhythm, and this accumulation will be useful. I just thought I could learn to edit and play my little role."

　

Thanks to her experience as an actor, Yang Xingyuan switched to editing, and she can better handle the rhythm of performances and shots.

　

When cutting "Man to Middle Age", because Lu Wenting and Yang Xingyuan are both of the same generation, Yang Xingyuan said that she edited the character completely according to the character and her own feelings. "Lu Wenting went to see the child, and the editor must understand how she looked at it in order to impress the audience. So I chose her to post it in the nearest place to watch the child sleeping, so that I can understand the psychological feelings of this character. "

With more than 40 years of experience in behind-the-scenes editing, Yang Xingyuan concluded that editors should think about editing from three angles.

　

The first is the director. "I must know what he wants to do, what he wants to show and what he wants to give to the audience." Then there is the character, "to understand the character’s mentality and his various things."

Then there is the audience. "You must know what the audience wants to see and where his interest lies. I can’t say that I am an old lady now. My rhythm is slow, so I don’t appreciate the audience’s appreciation ability. I also deal with the film according to my rhythm and speed, and the audience will definitely stop watching it. "

Daughter Sun Yiqun did a lot of work that had nothing to do with art. Later, her mother thought she should learn a skill, so she taught her to edit and took her into the business. The two later jointly edited the TV series Behind the Vanity.

　

"They always ask why the play they cut is not good-looking. I said that because you cut the process, it is not a play." Sun Yiqun believes that a good editing is to "pick up" the scene and find the eye of the scene, while a bad editing is to exert an average force. "If the scene and the scene are not more energetic, it will not work."

　

In her view, editing and scriptwriting have a lot in common, they all want to tell stories, and they all want to think about how to make this story vivid, interesting and arresting.

From Yang Xingyuan to Sun Yiqun, from film editing to digital editing, the editors of mother and daughter have great enthusiasm for this art, which has been passed down from generation to generation not only for the iterative upgrade of film editing skills, but also for the consistent dedication, concentration, lean and innovative craftsmanship as editors.

Country Garden said that the company is actively taking self-help measures, trying its best to increase revenue and reduce expenditure, and concentrate resources to ensure delivery.

Reduce expenses: the annual salary of the four directors is reduced to 120,000 yuan, and the administrative expenses are reduced by 60%.

On December 12th, Country Garden Holdings Limited announced that the directors’ salary was adjusted.

According to the announcement, in combination with the actual business needs of the industry and the company, Ms. Yang Huiyan, Mr. Mo Bin, Ms. Yang Ziying, executive directors of Country Garden, and Mr. Chen Nai, non-executive directors, offered to reduce their salaries, and signed a supplementary agreement and a supplementary letter of appointment with the company on November 1, 2023, which will be the latest annual salary adjustment for directors as follows (adopted by the board of directors of the company on the date of this announcement and effective on November 1, 2023): Yang Huiyan will be adjusted from 370,000 yuan to. Mo Bin was adjusted from 3 million yuan to 120,000 yuan; Yang Ziying was adjusted from 2 million yuan to 120,000 yuan; Chen Tai was adjusted from 370,000 yuan to 120,000 yuan.

It is understood that Country Garden has made several adjustments to executive compensation in the past two years.By 2023, executive compensation will drop by 86% compared with 2021.These executives include the vice president of the company headquarters, the general manager of the functional center and above, and the regional president.

According to Country Garden’s official micro-display, the whole group tried its best to reduce administrative expenses, all of which were based on the principle of zero, and unnecessary expenses were not spent. Rigid expenses were reduced as much as possible through integration and intensification. In 2023, administrative expenses were significantly reduced by nearly 60% compared with 2021.

At present, Country Garden has cancelled all senior executives’ car allocation. When there is a real need for work, it can achieve intensive vehicle cost control and cost reduction by applying for official car allocation or using its own car. For all group buses including the former senior executives’ car allocation, assets are revitalized through internal public bidding and foreign workers’ debt repayment. In addition, including the reimbursement of senior executives’ medical examinations and the free canteen for senior executives, etc. have also been cancelled.

Streamlining organizational structure: 14 to 7

Recently, Country Garden has adjusted its organizational structure and merged 14 regional companies into 7 new regions: Hebei and Beijing merged into North China; Shandong and northern Jiangsu merged into Lusu region, northwest and Shaanxi merged into central and western regions, Anhui and Zhejiang merged into Zhejiang-Anhui region, Jiangxi and Fujian merged into Jiangxi-Fujian region, Yunnan and Sichuan merged into Sichuan-Yunnan region, and Guangxi and Jiangzhong merged into Jiangzhong region.

In this regard, Country Garden said that under the current industry situation, this adjustment is aimed at further improving the adaptability between the organization and the business, promoting flat and efficient management, complementing the available resources, and doing their best to ensure the realization of the strategic goal of "guaranteeing delivery".

Yang Huiyan said at the management meeting that the overall organizational structure of the company has reached a relatively stable stage after certain adjustments at the group headquarters and regional project level. What we need to do next is to lock in outstanding talents, adhere to the open and transparent mechanism of selecting and employing people and cultivating talents, and better improve the short, medium and long-term salary and incentive mechanism.

Guaranteed delivery: It is estimated that the delivery will exceed 400,000 sets next year.

Country Garden said that ensuring delivery is the company’s vital corporate responsibility and the safety bottom line of the property market.

According to public information, in 2022, Country Garden Group and its joint ventures and affiliated companies delivered nearly 700,000 houses, ranking first in the industry in terms of delivery volume. In the first 11 months of 2023, the Group has delivered more than 500,000 houses with a total delivery area of 60.43 million square meters, involving 240 cities in 31 provinces (municipalities and autonomous regions), and the delivery volume once again ranks first in the industry.

At the management meeting of Country Garden Group in December, Yang Huiyan pointed out that in the next 12 months, the delivery challenge will enter the deep water area.The delivery volume of Country Garden is expected to exceed 400,000 sets in 2024.The number is still huge. "Guaranteeing delivery is a very difficult but also very important task. I hope that everyone will be ready, attach great importance to it and do a good job in pre-planning."

Open source: "selling hematopoiesis"+"two wings" business

At Country Garden Group Management Meeting in December, Yang Huiyan, Chairman of the Board of Directors of the Group, said:"The family will definitely support the company by selling iron, and the company will strive to explore an effective path to resume normal operations as soon as possible."

It is reported that, as the controlling shareholder, Yang Huiyan family has supported a total of about HK$ 41 billion by borrowing money, increasing stock holdings, purchasing bonds, and paying interest by shares. Among them, HK$ 1.56 billion interest-free unsecured loans were provided to the Group in 2021, and HK$ 5.05 billion was raised in December 2022 for interest-free unsecured loans to the Group.

(1) Promote sales and realize hematopoiesis.

Recently, the management and financial department of Country Garden Group conducted a sensitivity simulation analysis of the extreme situation that the company may face in its operation. Yang Huiyan said: "Under conservative calculation, as long as our inventory assets are sold normally, Country Garden will continue to maintain positive assets in the next decade even assuming that we don’t buy land, there is no tax reduction, and the new contribution of the two wings is not considered."

Mo Bin, president of Country Garden, said that what Country Garden is doing now is mainly hemostasis and blood transfusion. At the same time, it is necessary to cultivate internal strength, get through difficult times, and strive to increase sales and realize hematopoiesis, so that enterprises can truly return to the track of healthy development. In addition, Mo Bin stressed that in order to realize sales hematopoiesis, first, we should grasp the key sales nodes in December, increase the renovation of existing buildings, mobilize national marketing, strive to break through special revitalization and promote the return of cash. Second, we should pay close attention to the payment at the end of the year and do a good job in collecting overdue receivables at the end of the year. At the same time, it is necessary to plan the work of returning home during the Spring Festival in advance, and the returning areas should make full preparations in advance to prepare for a good start in supply and sales arrangements.

However, the current sales situation of Country Garden is still not optimistic.From January to November, 2023, the cumulative sales of Country Garden was 212.45 billion yuan, down 52% year-on-year, which was the largest year-on-year decline among the TOP30 housing enterprises. In addition, the monthly sales of Country Garden are still in a downward trend: since July this year, the sales of Country Garden have dropped sharply, of which the contracted sales in June was 16 billion yuan, which fell to 12.07 billion yuan in July, 7.98 billion yuan in August and 6.17 billion yuan in September respectively. In October, the sales volume was 6.31 billion yuan, compared with the previous month, but the latest data showed that the sales volume showed a downward trend again, and the sales volume in November was 6.11 billion yuan.

(2) Cultivate new business growth points

In addition to the real estate sector business, Country Garden is also making efforts in technology construction and escrow construction. It is reported that since June this year, Bozhilin robot under Country Garden has started to sell to the market. At present, it is mainly leased to buy and repurchased by old customers. In terms of agency construction, Phoenix Zhituo has added four new projects, including Qinhuangdao in Hebei, Luoyang in Henan, Shangqiu in Henan and Yancheng in Jiangsu, on the basis of the original projects since its establishment two months ago. Country Garden said that it will continue to cultivate new business growth points in the future.

(This article first appeared in Titanium Media APP, author | Wang Jian, editor | Liu Yangxue)

For more macro research on dry goods, please pay attention to titanium media international think tank WeChat official account:

Data map: Taiyuan, Shanxi, postal workers are sorting express delivery. China News Service reporter Zhang Yunshe

　　BEIJING, Nov. 12 (Xinhua) According to the official WeChat news of the State Post Bureau, on November 11, according to the monitoring data of the State Post Bureau, postal and express delivery companies handled 535 million express mail all day, which was three times the daily processing volume since the second quarter, with a year-on-year increase of 28.6%, a record high.

　　It is estimated that the peak season of "double 11" this year will last from November 11th to 18th. During the peak period, the whole industry will handle 2.8 billion pieces of express mail. The whole industry still regards "peak-shifting delivery and balanced promotion" as the core mechanism, and strictly controls the peak delivery volume according to the delivery carrying capacity of each enterprise and the planned push volume. Through big data platforms such as postal industry safety supervision information system and e-commerce collaborative platform, the State Post Bureau will pay close attention to the whole network operation in real time, guide enterprises to control the dispatching rhythm, and moderately extend the delivery time limit of double 11.

　　In order to achieve the goals of "two noes" (no paralysis of the whole network and no explosion of important nodes) and "three guarantees" (smooth flow, safety and stability), on the basis of the existing service capacity, the whole industry temporarily hired nearly 400,000 people and 120,000 vehicles, and added more than 3.6 million square meters of processing sites and more than 3,000 sets of automatic and semi-automatic sorting equipment. Technically, unmanned warehouses, drones and unmanned vehicles have gradually achieved mass production and large-scale application. More than 200 intelligent distribution centers have been built nationwide, and many unmanned warehouses have been set up by enterprises such as SF, JD.COM, Cainiao and Suning. A large number of heavy-duty storage robots and distribution robots have been promoted and applied in different occasions, and the "illegal operation AI intelligent identification system" that automatically identifies violent sorting behavior has been put into operation in some enterprises. In urban areas, 82,000 terminal public service stations have been built nationwide, and more than 380,000 sets of intelligent express boxes have been deployed, effectively supplementing the terminal service capacity of express delivery. The establishment of 59,000 rural express public pick-up and delivery points will better meet the delivery service needs of rural residents. In terms of international business, the outlets of express delivery enterprises in more than 60 countries, overseas warehouses covering more than 50 countries and regions and new international routes will play a very positive role. In terms of after-sales, AI intelligent customer service robots that can achieve 24-hour service have been put into use in many enterprises, which will effectively improve customer service capacity during peak hours.

　　In response to the consumer’s concern about the "last mile" in rural areas in the peak season, the relevant person in charge of the State Post Bureau pointed out that up to now, express outlets have covered more than 30,000 towns and villages, and the coverage rate of express outlets in towns and villages nationwide has reached 96%, effectively improving the distribution capacity in rural areas. At the same time, the State Post Bureau specially issued the "Implementation Plan for Accelerating Express Delivery to the Countryside" this year, intensified efforts to build a terminal delivery service system consisting of home delivery, box delivery and platform delivery, and constantly promoted the integrated development of transportation and postal express delivery, and promoted the coordinated development of express delivery and rural e-commerce. In addition, since April this year, two rounds of cleaning up and rectifying illegal charges for express terminal services have been deployed nationwide, effectively safeguarding the legitimate rights and interests of farmers. On the issue of express packaging, with the help of "double 11", the industry has vigorously promoted the "9571" project of green development, continuously reduced the secondary packaging of e-commerce express mail, and deployed 27,000 standardized express packaging recycling devices at terminal service stations. At the same time, enterprises have done a lot of work in reducing the use of packaging materials, promoting electronic face sheets, circulating transfer bags and packaging recycling boxes.

Published by:

Li Jun ‘an is a member of the Party Group of Zhengzhou Municipal Market Supervision Administration, a second-level senior sponsor and a spokesperson.

Zhou Fang, Head of Food Production Supervision Department of Zhengzhou Municipal Market Supervision Administration

Mao Lixin Deputy Secretary of the Party Committee of the Law Enforcement Inspection Detachment of Zhengzhou Municipal Market Supervision Administration

Luo Xiangyu Director of Zhengzhou 12315 Complaint Reporting Rights Protection Center

Deputy Secretary General of Zhengzhou Consumer Protection Association, Chen Xiaoyan

Moderator:

Yang Xin, Director of the Press and Publicity Department of Zhengzhou Municipal Market Supervision Administration

Time: March 10th, 2021 at 10:00.

Moderator Xin Yang

Friends of the press, distinguished guests,

Good morning!

Welcome to today’s press conference.

The annual "March 15th International Consumer Rights Day" is coming soon. This year, the theme of Consumer Rights Protection Year announced by China Consumers Association is "Protecting Safe and Smooth Consumption", which is intended to strengthen the supervision responsibility of government departments and make every effort to create a safe consumption environment. Around this theme, combined with the responsibilities of the market supervision department and the consumer association, our supervision department and the Municipal Consumers Association summarized the analysis report on the acceptance of consumer complaints and reports in 2020 and the development of law enforcement inspection in 2020, and announced it to the public.

First of all, let me introduce the leaders and guests attending today’s press conference:

Li Jun ‘an, member of the Party Group of Zhengzhou Municipal Market Supervision Administration, second-level senior sponsor and spokesperson; Zhou Fang, head of the Food Production Supervision Department of Zhengzhou Municipal Market Supervision Administration; Mao Lixin, deputy secretary of the Party Committee of the Law Enforcement Inspection Detachment of Zhengzhou Municipal Market Supervision Administration; Luo Xiangyu, director of the 12315 Complaint Reporting Rights Protection Center; and Chen Xiaoyan, deputy secretary-general of Zhengzhou Consumer Protection Association.

Also present at today’s press conference are friends from the central, provincial and municipal media. Welcome everyone!

Next, please invite Li Jun ‘an, a member of the Party Group of Zhengzhou Municipal Market Supervision Administration, a second-level senior sponsor and a spokesperson, to release an analysis report on the acceptance of consumer complaints and reports in 2020.

Li junan

Media friends:

Good morning everyone!

The Party Group of Zhengzhou Municipal Market Supervision Administration attaches great importance to the protection of consumers’ rights and interests. In 2020, according to the reform plan and work requirements of market supervision institutions, the Municipal Bureau established a new Zhengzhou 12315 Complaint Reporting Rights Protection Center. With the number of "12315", we provided market supervision consultation, complaint and report services to the outside world, and opened 30 telephone seats to provide 24-hour hotline service for consumers in our city throughout the year. At the same time, the Municipal Bureau focuses on serving people’s livelihood, insists on keeping integrity and innovation, and actively builds a consumer rights protection network. In all major enterprises and supermarkets in the city, a "consumer rights protection service station" has been established, and enterprises have been mobilized to actively solve "online consumption disputes", organized "safe consumption creation" activities, and promoted the commitment mechanism of "no reason to return goods offline". Through various efforts, the city’s consumer market has been further purified, the market consumption environment has been optimized, and the sense of gain and happiness in the consumer environment has been improved.

In 2020, the city’s 12315 system received 413001 consumer inquiries, complaints and reports, including 258168 inquiries, 81800 complaints and 73033 reports. 81,593 complaints were settled, with a settlement rate of 99.74%. The amount of disputes involved in the complaints was 334.1707 million yuan, and the amount of economic losses recovered was 72.5416 million yuan. 72,743 reports were completed, with a completion rate of 99.6%.

Judging from the way of acceptance, the 12315 hotline received 325195 consumer appeals this year, and it is still the main channel for complaint acceptance; The national 12315 Internet platform received 61436 consumer appeals, which was the second acceptance channel; The mobile client, WeChat, Alipay applet and mobile APP received 21,026 consumer appeals, and the receiving volume increased significantly. There were 5,344 incoming letters and other forms, and the proportion of receiving letters decreased compared with previous years, indicating that the trend of consumer complaints is developing in the direction of convenience, digitalization, networking and intelligence, and also embodying the advantages of "internet plus" government services.

Based on the summary analysis of hot issues of consumer complaints in 2020, it is mainly reflected in the following five aspects:

I. Complaints and reports on residents’ services and daily necessities

A total of 8959 such complaints and reports were accepted throughout the year. First, fitness and sports venues. The problems of recharging and refunding fees caused by the epidemic situation, such as not being able to operate normally, changing business address, leaving private education, etc., mainly include "it is easy to apply for a card, it is difficult to return the card", "closing the store and running away", "restricting consumption" and "standard terms". The second is clothing, shoes and hats. There are many disputes about return and exchange caused by inconsistent ingredients, quality defects, physical differences and after-sales service. The third is a cultural communication company. Tickets are sold online, but affected by epidemic control or other reasons, which leads to the cancellation of performances or disguised price increases, and it is difficult for consumers to refund tickets. The fourth is household items. The quality and specifications of the physical object are inconsistent with the samples, the goods are not delivered in time, and the appearance defects are difficult to return.

Second, food complaints and reports

A total of 8996 such complaints and reports were accepted throughout the year. First, frozen and quick-frozen foods. Problems such as foreign body, mildew and deterioration of food are relatively concentrated; Second, merchants fictionalize and exaggerate the efficacy of food. There are problems such as false propaganda and illegal propaganda; The third is the labeling of food packaging. There are problems such as incompleteness, failure to indicate the name and address of the factory, no production date and no production license number; The fourth is to order food online. Problems such as non-delivery, less delivery, non-payment and substandard hygiene of catering units.

Iii. Complaints and reports on vehicles

A total of 2728 such complaints and reports were accepted throughout the year. The first is the problem of car purchase and consumption. After the intention to buy a car, the merchant promised to get a refund, but in the end, it was impossible to get a refund, the consumer could not pick up the car within the promised time, and the 4s shop did not agree to change the model, which made it difficult for consumers to get a refund. The second is the issue of fees. After the repayment of the car by installment is completed, the problem of charging the handling fee for releasing the charge; The third is vehicle maintenance. During the warranty period, vehicle maintenance, on the grounds that there are no accessories, will not be repaired or buck passing, as well as the abnormal quality of the car after the three-guarantee sale.

Four, household appliances and communication equipment complaints.

A total of 2,874 such complaints and reports were accepted throughout the year. The first is the quality of household appliances. Purchase, installation and delivery of household appliances, quality or defects, and damage after use; The second is the after-sales service of household appliances. The main problems are no maintenance, lack of accessories, no return and other issues; The third is the distinction of after-sales responsibility. No "three guarantees" on the grounds of man-made damage and unclear definition of maintenance responsibility; Fourth, it is difficult to return communication equipment. It is suspected that the purchased mobile phone is a refurbished or second-hand mobile phone, and it is difficult to communicate and collect evidence and return it; The fifth is the problem of mobile phone accessories. Mobile phone repair and replacement of second-hand accessories, the use of counterfeit and inferior mobile phone accessories and other issues.

V. Complaints and reports on decoration and building materials

A total of 948 such complaints and reports were accepted throughout the year. Mainly reflected in the decoration materials and cabinets, doors and windows, furniture and other issues. The first is advertising. Merchants use absolute terms such as "best", "highest quality" and "leading technology" in advertisements, and use exaggerated propaganda and false propaganda such as "never fading", "nontoxic and environmentally friendly" and "antibacterial and breathable". The second is the issue of service commitment. After-sales service has problems such as deliberate delay or unreasonable refusal, failure to deliver goods on time, failure to install on time, failure to fulfill service commitments, etc. The third is product quality. There are some problems in building materials, such as unqualified quality, fake materials, fake and inferior quality, and excessive odor.

In order to effectively strengthen the protection of consumers’ rights and interests, and smooth the channels for reporting complaints, the Municipal Bureau has arranged to launch a special campaign to ensure safety and stability in the whole city in early March. Focus on key areas, key places, key units, key equipment and key areas to carry out centralized management, eliminate potential safety hazards and protect consumer safety. During the special action, the general public are invited to actively participate. If potential risks in the market consumption field are found, or infringement of consumers’ rights and interests is infringed, complaints can be made by calling the "12315" hotline; You can also report complaints through the National 12315 Internet Platform (www.12315.cn).

Dear friends, 2021 is the centenary of the founding of the Communist Party of China (CPC), the first year of the 14th Five-Year Plan, and a crucial year in the process of the modernization of Zhengzhou as a national central city. At present, it coincides with the National People’s Congress and the "March 15th" International Consumer Rights Day. The city’s market supervision system will conscientiously implement the "four strictest" requirements put forward by the Supreme Leader General Secretary, improve political stance, highlight problem orientation, safeguard consumer safety, and serve people’s livelihood. We will severely crack down on illegal acts that infringe on consumers’ rights and interests according to law. Escort consumers with excellent results, show a good image of market supervision, promote the high-quality development of Zhengzhou, and present gifts to the centenary of the founding of the Communist Party of China (CPC).

Thank you!

Moderator Xin Yang

Next, please ask Mao Lixin, deputy secretary of the Party Committee of the Law Enforcement Inspection Detachment of Zhengzhou Municipal Market Supervision Administration, to release the development of law enforcement inspection in 2020.

Mao Lixin

In 2020, we thundered to crack down on violations of laws and regulations in the field of market supervision, and the special rectification achieved remarkable results. Help prevent and control the epidemic, focus on the prices of daily necessities such as vegetables, and decisively dispose of the case of "high-priced cabbage", which won praise from netizens all over the country and effectively stabilized the market price. Organize special rectification of epidemic prevention supplies certification, and handle 26 inter-provincial transfer cases. Special enforcement of advertising was carried out, and 337 false and illegal advertisements were closed, with a fine of 6.177 million yuan. Xiangsheng Street Store, a Pingxin pharmacy in Henan Province, issued a false advertising case, which was listed in the "Typical Cases of National False and Illegal Advertisements in 2020" by the General Administration. 9 cases of medical services and education fees were handled, with a fine of 639,000 yuan. In the city, 2,960 cases of medical equipment, cosmetics and food hygiene were filed, 2,454 cases were closed, 21.002 million yuan was fined, 25 cases were handed over to the public security, 15 cases were filed, 7 people were detained and 7 people were arrested. 35 cases of illegal salt were settled. The tobacco market banned 481 unlicensed merchants, handled 2,249 cases with a case value of 44.627 million yuan, fined 4.235 million yuan, handed over 136 cases to public security organs, detained 27 people, arrested 6 people and sentenced 11 people. We cracked down on "counterfeiting and confusion" and unfair competition in key areas, handled 36 cases and fined 693,000 yuan. Strictly crack down on illegal pyramid selling activities, put on record 3 cases, and educated and dismissed 51 pyramid selling personnel.

In 2021, we made great efforts to prepare for war, integrated the law enforcement and inspection functions of the original six major areas of industry and commerce, quality supervision, food and medicine, price, intellectual property rights and salt law enforcement, set up a comprehensive law enforcement and inspection detachment, adhered to the leadership of the party, adhered to the people-centered, adhered to administration according to law, practiced the "four strictest" general requirements, clenched our fists, polished our swords, and were fair, just, open and civilized.

Moderator Xin Yang

Next, please ask Chen Xiaoyan, Deputy Secretary-General of Zhengzhou Consumers Association, to issue a consumption reminder for 2021.

Chen Xiaoyan

In 2020, Internet service complaints ranked the third among all kinds of complaints accepted by Zhengzhou Consumers Association in the whole year, including 834 complaints and inquiries involving Zhengzhou Branch of Great Wall Broadband Network Service Co., Ltd. (hereinafter referred to as "Great Wall Broadband"). The main problems that consumers reflected on "Great Wall Broadband" were: First, poor network signal; Second, the refund time is long, and the phenomenon of delaying and shirking is serious; Third, the calculation of refund is unreasonable; Fourth, in the case that broadband services cannot be guaranteed, we will continue to induce uninformed consumers to pay broadband fees. In response to consumer complaints, Zhengzhou Consumers Association has repeatedly talked about "Great Wall Broadband" and urged it to introduce solutions as soon as possible. Up to now, a large number of consumer complaints have been delayed.

Zhengzhou Consumers Association believes that "Great Wall Broadband" company’s internal management is lax, its service concept is not correct, its awareness of safeguarding consumers’ rights and interests is weak, and its principle of good faith management is weakened, which fails to fulfill its legal responsibility as the first person responsible for safeguarding consumer rights and interests, resulting in the infringement of consumers’ right to know, fair trade and claim. Therefore, Zhengzhou Consumers Association reminds consumers: In view of the fact that "Great Wall Broadband is easy to pay and difficult to protect rights", please carefully choose "Great Wall Broadband" service.

In addition, we should pay attention to the following points when choosing broadband services: First, we should choose carefully. Go to a regular, reputable and complete after-sales service communication operator, and don’t believe in small advertisements posted at roadside agents or on the street, as well as broadband services sold at home. The second is to pay attention to retaining contracts and consumption credentials. Sign a written contract, and don’t trust verbal promises easily. When signing a contract, you should carefully read the terms of the contract, ask for an invoice in time after payment, keep relevant consumption and transaction credentials, and try to avoid transferring service fees to personal accounts through WeChat or Alipay. The third is to protect rights according to the law. When consumers encounter disputes, they can safeguard their legitimate rights and interests by negotiating with operators, asking consumers’ associations for mediation, complaining to relevant administrative departments, and bringing a lawsuit to the people’s court.

Moderator Xin Yang

Next, please ask reporters to ask questions about your concerns. Before asking questions, first inform your unit.

Dahe. com: In recent years, with the rise of live webcasting, there are more and more online shopping disputes. Can you briefly introduce this situation?

Luo Xiangyu

Online shopping is now a favorite consumption mode of our consumers, especially the rise of live webcasting with goods in recent two years, which has further promoted the network economy. But in fact, there is no big difference between webcasting and online shopping, both of which belong to remote shopping. Due to the rise of such remote shopping, consumer complaints are increasing day by day.

Taking 2020 as an example, the city’s 12315 system accepted about 15,000 online shopping complaints, accounting for nearly 20%. The main complaints are as follows: First, some anchors or platforms have exaggerated or false propaganda problems. If the goods are falsely described, the function and utility of the products are exaggerated; Promised concessions, gifts, etc. Second, some e-commerce operators delay or refuse to perform the contract. Such as arbitrarily cutting orders and refusing to deliver goods, and selling goods that are not on the right board. Third, some e-commerce operators do not undertake after-sales service obligations. If you don’t implement the "seven-day return without reason" rule and don’t fulfill the "three guarantees" obligation, etc.

According to the Law on the Protection of Consumers’ Rights and Interests and the Measures for the Administration of Online Transactions, if consumers buy goods or receive services through online trading platforms, and their legitimate rights and interests are damaged, they can complain to the platform merchants and also to the third-party trading platforms, so consumers should first negotiate with the merchants or third-party platforms through themselves, and handling consumer disputes is also the legal obligation of the merchants and third-party platforms; If negotiation fails, consumers can complain to the market supervision department where the platform merchant is located, or the market supervision department where the third-party trading platform is located, call the local 12315 or make a complaint through the "National 12315 Internet Platform", and the market supervision department will accept it as soon as possible and deliver it to the jurisdiction for mediation of consumer rights protection. At the same time, violations of laws and regulations found in the process of online transactions can also be reported to the market supervision department, which will be investigated and verified by the jurisdiction and dealt with according to law.

Consumers are also reminded to choose formal and qualified e-commerce platforms and sellers with high credibility when shopping online; Before placing an order, you should read the product details, promotion rules, return and refund methods and after-sales service policies, etc. Don’t trust the advertisements of merchants or the sensational agitation of anchors with goods, and avoid impulsive consumption and passionate consumption. At the same time, when purchasing, you must choose a formal trading platform, inquire about the business qualifications of the business operators, understand the true attributes of the purchased goods, try to keep relevant transaction records, publicity records, service commitments, etc., and keep sales vouchers to avoid the inability to prove when problems arise. After a dispute arises, try to settle it through consultation. If it fails, you can complain to our market supervision department or consumer association, and our market supervision department will do its best to safeguard the legitimate rights and interests of consumers.

Hong Kong Economic Herald: 3.15 is coming soon. Can you remind consumers of the problems that should be paid most attention to in safeguarding their legitimate rights and interests according to the daily complaints received by consumers?

Chen Xiaoyan

According to the analysis of daily complaints, especially the cases of difficulties in safeguarding rights, we notice that if consumers can improve their awareness of safeguarding rights and self-protection, pay more attention to pre-consumption claims and conscientiously perform inspection responsibilities, then even if they encounter consumer disputes, whether they negotiate with operators to safeguard their rights or seek help from consumer associations or relevant departments, consumers who are "weak" can provide more powerful assistance for the settlement of consumer disputes, which is convenient for relevant departments to help consumers solve consumer disputes.

For example, we recently received a complaint: when a consumer bought food in a supermarket, the name of the food printed on the consumption certificate provided was inconsistent with the food he bought, and the price was also inconsistent. Such proof affected the consumer’s right to claim compensation after being infringed. In the consumption survey we carried out a few years ago, we also found that the name of the goods on some supermarkets’ printed receipts was inconsistent with the goods actually purchased. In this case, if consumers did not check carefully on the spot, they would not be able to provide effective proof in time when they suffered personal and property losses.

At present, consumers should pay attention to the consumer complaints when we buy goods or receive services in our daily life: for commodity consumption, we should take the initiative to obtain and keep the consumption credentials when we consume, and at the same time, we should pay attention to checking the machine-made receipts and invoices provided by operators on the spot to ensure that the names of the bills are consistent with the names of the purchased goods, the specifications and models are consistent, and the sales prices are consistent with the personal payment prices. For service consumption, such as beauty salons, body building, home improvement, education and training, accommodation and tourism, etc., in addition to paying attention to actively obtaining and retaining consumption credentials, we should sign a service agreement with the operator at the same time, and indicate or sign a supplementary agreement in the format contract for the personalized commitment service of the operator. Prevention in advance is a strong support for safeguarding rights after encountering problems!

This year, the City Consumers Association will also strengthen consumer publicity and education, carry out in-depth consumer publicity and education into community activities, send laws and regulations, rights protection methods and consumer warning tips to consumers, and make continuous efforts to improve the consumption environment in our city!

Elephant News: A few days ago, some media reported clues about the problem of bottled water. What measures did our regulatory authorities take? Can you introduce the specific situation of the investigation?

Zhou Fang

Municipal Market Supervision Bureau has always attached great importance to food safety supervision, including bottled water, and organized special rectification, supervision and sampling inspection of bottled water production for many years. The production conditions, food safety control ability and quality and safety level of bottled water production enterprises in the city have been continuously improved. On the morning of February 6th, the online media of Dongfang Jinbao published an article on "Insider Investigation of Bottled Water Fraud", which reflected that the illegal production and operation of bottled water in the market had not been completely eradicated. After learning the relevant public opinion information, the leaders of the Municipal Bureau attached great importance to it and acted quickly that morning to organize and investigate the relevant clues reflected by public opinion. Even during the Spring Festival holiday, the investigation of relevant clues was not relaxed, and the promotion meeting was held several times in a row to arrange the deployment, and the front-line supervision was organized to ensure that the investigation and rectification were in place.

On February 6th, the online media of Dongfang Jinbao reported that the main clues were that water shops directly sold tap water and groundwater privately. Individual bottled water sales points have poor sanitation; Individual bottled water products have no identification information such as manufacturer and address; There are hidden quality problems in barreled water buckets and lids.

In response to the clues reflected by the media, our bureau immediately set up a special working class, which is personally responsible by the main leaders and composed of relevant personnel such as food supervision, news propaganda, law enforcement inspection and the public security team stationed in the bureau. According to the problems and clues reflected by the media, we went to Jinshui District, Economic Development Zone, Guancheng District, Airport District, xinzheng city, zhongmou county and other places to conduct investigations one by one with the regulatory authorities of the jurisdiction, and investigate and deal with illegal acts in time.

After investigation, Aoquan bottled water store in Zhengzhou Economic Development Zone illegally filled bottled water without any logo without obtaining the food production and operation license. The store is suspected of violating the relevant provisions of the Food Safety Law and engaging in food production and business activities without permission. The market supervision department of Zhengzhou Economic Development Zone has filed a case for investigation and punishment according to law, and made a penalty decision of confiscating 165,300 yuan. Currently, relevant administrative penalty procedures are being implemented.

The manufacturer of the "Yuzhiyang" bottled water involved in the report is Henan Yuzhiyang Beverage Co., Ltd., and its production address is Houwei Village, Xie Zhuang Town, Xiangyun Road Office, Zhengzhou Economic Development Zone, and it has not obtained the Food Production License. The company is suspected of violating the relevant provisions of the Food Safety Law and engaging in food production activities without permission. The market supervision department of Zhengzhou Economic Development Zone has filed a case according to law, and based on the illegal facts and illegal income, it has made a penalty decision of confiscating 250,200 yuan, and is currently fulfilling relevant administrative punishment procedures.

Sun Ping ‘an Department Store in Mengzhuang Town, xinzheng city did not fulfill the obligation of incoming inspection when purchasing, and operated bottled drinking water with labels that did not meet the requirements. The market supervision department of xinzheng city ordered the parties to correct the illegal behavior, and gave them an administrative penalty of warning, confiscation of illegal income of 507.5 yuan and fine of 10,000 yuan.

Shigengxin bottled water service station in Jinshui District, Lanpei bottled water service station and Lantian water station in No.135 Buchang Street, Guancheng Huizu District have not obtained food business licenses, and the local market departments have ordered them to apply for registration certificates of small food shops within a time limit.

Other problems reflected by the media, food safety violations have not been found in the field verification.

In order to further strengthen the supervision of barreled drinking water production and business operation units and effectively ensure the food safety of the people, on February 10th, our bureau issued the "Special Remediation Plan for Barreled Drinking Water Production and Business Operation in 2021", arranged and deployed the special rectification work of barreled drinking water production and business operation for 10 months, and adopted measures such as special inspection, comprehensive sampling inspection, intensified disposal, and informing industry organizations to strengthen self-discipline, so as to standardize the production of barreled drinking water, severely investigate and punish unlicensed production, sales and illegal filling without permission. Since the implementation of the rectification, the city has dispatched more than 1,000 law enforcement officers and 383 law enforcement vehicles, inspected 720 bottled water production and business units, sampled 241 batches of bottled water and source water samples, found 35 problems, ordered 35 rectifications, put 4 cases on file for investigation, and banned 10 production sites suspected of illegally producing bottled water in conjunction with local governments.

In the next step, we will also give full play to the role of complaint reporting and unblock all kinds of complaint reporting channels such as 12315. Give full play to the management advantages of township (street) party committees and governments, such as being close to the masses, gridding, long-distance system and long-distance system, solve the problems of "black dens" in food production and operation, strong concealment of illegal and criminal acts, and difficulty in daily supervision, establish a long-term mechanism of "whistling at the grass-roots level and responding by departments" to find and deal with problems, and effectively improve the food safety level of the whole city.　

Moderator Xin Yang

Due to the time, today’s press conference is over. If you need to continue the interview after the meeting, please contact the department in charge of news propaganda of Zhengzhou Municipal Market Supervision Administration at 67886297.

Today’s press conference is over, thank you!

　　In practice, a large number of inventions are protected by submitting patent applications and obtaining authorization. Therefore, the writing quality of patent application documents often affects whether the patentee can successfully defend his rights and resist competitors. In this paper, the author discusses the writing skills of patent application documents from the perspective of writing patent application documents.

　　First, we should consider the appropriate scope of protection. The so-called "appropriate" scope of protection refers to obtaining as large a scope of protection as possible on the premise of complying with China’s patent law and its implementation rules.

　　The author thinks that a patent application document can "predict" the comparison documents that may be retrieved, which is based on the full retrieval of domestic and foreign patent documents in the early stage of writing the patent application document, so as to determine the appropriate level of existing technology, construct an appropriate scope of protection, and make the patent more stable.

　　In practice, the author found that many existing patent application documents only describe the technical scheme itself. The author believes that the process of writing patent application documents is a process of secondary creation, which requires the joint participation of inventors and patent agents to discuss various implementation methods to solve the technical problems involved in invention-creation, and determine the claims after summarizing and abstracting the technical characteristics of the implementation methods (upper generalization or functional limitation). And the upper generalization also needs as many examples as possible so that the claims can be supported by the specification. When judging the infringement, the functional features are interpreted as the specific embodiment disclosed in the specification and its equivalent embodiment, and the application of the equivalence rule is easy to have objections. Therefore, it is helpful to obtain a more appropriate scope of protection by excavating as many embodiments as possible in the writing stage of patent application documents.

　　Second, when writing patent application documents, we should also consider it from the perspective of being conducive to infringement judgment. For example, based on principle of universal coverage, when writing the patent application file at first, only the most necessary technical feature set that can solve the technical problems of the invention needs to be written into the independent claim, and unnecessary technical features will only limit the narrowing of the protection scope. In practice, the name of the invention, the characteristics of the use environment, the technical characteristics of the product defined by the preparation method, and the step sequence of the method claim may limit the scope of patent protection, so it should be carefully considered when using it. If there are possibly ambiguous terms in the claim and they are not explained in the specification, it may cause obstacles to the interpretation of the subsequent claims.

　　Third, the commercial prospects of patented products or methods should be considered. In the writing stage of patent application documents, patent agents may find that technical solutions can be applied to fields with more commercial prospects during the communication with inventors. For example, a technical scheme can be used in PC, but after understanding the existing technology, it is found that its application prospect in mobile terminal is very considerable, but it needs to be adjusted accordingly when applied in mobile terminal, so different technical schemes are produced, and the application strategy needs to be adjusted in time.

　　Fourth, we should consider whether patents can be easily evaded by competitors. The patent agent can work with the applicant to develop possible improved technical solutions and possible deteriorated technical solutions. The improved technical solutions can be used as a "retreat" in the patent examination stage and may prevent competitors from submitting improved solutions to patent applications. According to the Guide to Determining Patent Infringement issued by Beijing Higher People’s Court, the deteriorated technical scheme does not constitute equivalent infringement, so the deteriorated technical scheme should also be considered when writing patent application documents.

　　Fifth, it should be considered that it is conducive to obtaining evidence and safeguarding rights. If the applicant is engaged in the production of products, from the perspective of tort compensation, it may be more beneficial for the right holder to write patent application documents mainly against product manufacturers. This is because it is generally easier for sellers, importers and users of infringing products to prove the legal source of infringing products. In this case, it is advantageous to write the product and the claim of the preparation method of the product.

　　In the fields of communication, network and computer, the applicant may face multiple infringing objects, and which infringing objects he hopes to resist is an important factor in determining his claim. Different claims can be designed for different infringing objects.

　　Generally speaking, it is more beneficial to obtain evidence if you can know the technical characteristics of the product without disassembling it or using it by consumers. If you need reverse engineering to determine whether a product is infringing, you need to consider the difficulty of reverse engineering. For example, for related patents in the chemical field, the technical features that can be measured by ordinary measuring instruments or technologies are more conducive to obtaining evidence. For patents related to semiconductor and chip fields, if reverse engineering is easy to realize, components and their connection modes can be described. If reverse engineering is difficult to realize or the cost is high, it is necessary to consider whether it is necessary to change the writing method.

　　Sixth, we should consider the layout strategy. When writing patent application documents, we should also pay attention to the role of dependent claims, that is, in addition to independent claims, other novel and creative technical features can be arranged in dependent claims to prevent competitors from evading design.

　　At the same time, different levels of protection scope should be considered, and some patent application documents may be incompatible with certain technical features. At this time, independent claims with different protection scope can be considered to avoid giving up some protection scope due to modification. In addition, in some cases, the protection scope of the method claim can be different from that of the product claim, for example, the protection scope of the method claim can be greater than that of the product claim to achieve different levels of protection.

　　Chinese applicants need to consider the relevant laws and regulations of different countries when writing patent application documents if they want to arrange patents abroad. When submitting an international patent application through the Patent Cooperation Treaty (PCT), due to the relatively limited space for modification, it is especially necessary to strictly check the priority documents.

　　For example, when filing an American patent application, we should avoid too much self-admission and discussion about the existing technology. Because the U.S. Patent and Trademark Office has a strict review of Antecedent Basis, patent Enablement and Written Description, it should be extra cautious when writing priority documents. (Li Xingdi only represents the author’s personal views)
　

Author: Hongge comes from Medical Wechat WeChat official account.

　 　　 　　　Recent epidemic situation in ChinaIn the process of getting up, there are some situations that deserve our attention.the most importantIsFalse negatives in nucleic acid detection. Intercepting relevant news, it was found that 8 confirmed patients in Shijiazhuang on the 22nd were repeatedly negative for nucleic acid detection, and 5 confirmed patients in Shijiazhuang on the 24th were repeatedly negative for nucleic acid detection.

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　 　On the 22nd, there were 11 new confirmed cases in Shijiazhuang, including:

Case 1: Male, 52 years old, from Huangjiazhuang Village. On the evening of 21st.Ninth nucleic acid positive;

Case 2: Male, 13 years old, Niujiazhuang Village. On the evening of the 21st.Nine times nucleic acid positive;

Case 3: Female, 11 years old, Zhongyao Village. On the evening of the 21st.Nine times nucleic acid positive;

Case 4: Male, 49 years old, Nanqiaozhai Village. On the evening of 21st.Ninth nucleic acid positive;

Case 5: Male, 47 years old, Niujiazhuang Village. On the evening of 21st.Ninth nucleic acid positive;

Case 6: Female, 65 years old, Niujiazhuang Village. On the evening of 21st.Ninth nucleic acid positive;

Case 7: Male, 11 years old, grandson of Case 6. On the evening of 21st.Ninth nucleic acid positive;

Case 11: Male, 67 years old, Xiaoguozhuang Village, husband of Case 2 on January 7th. On January 3, it was transferred to centralized isolation medical observation; On the evening of 21st.11th nucleic acid detectionFinally positive;

On the 24th, there were 7 new confirmed cases in Shijiazhuang:

Case 1: Female, 65 years old, Nanqiaozhai Village. Centralized isolation on the 11 th.Nucleic acid after 9 times, 22 positive.

Case 2: Female, 4 years old, Nanqiaozhai Village. Centralized isolation on the 11 th.Nucleic acid after 9 times, 22 positive.

Case 3: Female, 4 years old, Mi Jie, Chang ‘an District. Centralized isolation on the 13 th.Nucleic acid after 8 times, 23 positive.

Case 4: Female, 64 years old, Xiaoguozhuang Village. Centralized isolation on the 10 th.After 11 nucleic acids, 24 positive.

Case 5: Male, 34 years old, Xiaoguozhuang Village. Centralized isolation on the 8 th.After 11 nucleic acids, 24 positive.

Case 6: 34-year-old female, Liujiazuo Village, was positive for nucleic acid on the 5th, was diagnosed as asymptomatic infection on the 6th, and was clinically confirmed on the 24th.

Case 7: A 28-year-old male, living in Yuhua District, went to work by car at Zhengding Airport on weekdays. He was diagnosed as asymptomatic infected with nucleic acid on the 5th, and was clinically confirmed on the 24th.

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Since the outbreak of COVID-19 last year, the topic of high false negative rate of nucleic acid detection has been the focus of attention. It has been reported that the positive rate of Covid-19 detection by fluorescence quantitative RT-PCR is only 30%-50% at present, which leads to extremely high false negative rate.

For Hongge, who doesn’t know much about the test, this paper analyzes the relatively high false negative test by searching articles and documents, and summarizes some reasons to discuss with you.

First, the incidence of COVID-19 suggests that it is highly possible to collect positive samples in a proper time.

Academician Yuan Guoyong’s team conducted this cohort study in Hong Kong Princess Margaret Hospital and Queen Mary Hospital. From January 22 to February 12, 2020, 23 hospitalized patients who were diagnosed as COVID-19 by laboratory examination of nasopharyngeal or sputum specimens were screened in the two hospitals.

A total of 173 respiratory specimens were collected from 23 patients. The researchers used RT-QCPR to determine the viral load in the samples. The results showed that the median viral load of respiratory tract samples was 5.2 log10 copies /mL (interquartile range was 4.1-7.0), and only 3 patients (13%) did not detect Covid-19 RNA in saliva samples.

The researchers observed that,The viral load in saliva was the highest in the first week after the patient developed symptoms, and then gradually decreased.Eight days after the patient developed symptoms, airway extracts were collected, and there was no obvious downward trend in viral load. Of the 21 patients who did not die,Seven people (33%) could still detect viral RNA after 20 days of symptoms, but this was not related to the severity of the disease.

▲ Changes of viral load in saliva (blue line) and airway extract (red line) of all 23 patients. Abscissa D- days after symptoms appear; S/E- number of saliva/airway samples.

AprilAccording to a study published in Nature Medicine, COVID-19 patients are most infectious before symptoms appear, and they begin to shed SARS-CoV-2 2 2-3 days before the first symptoms appear.

https://doi.org/10.1038/s41591-020-0869-5

The study, led by Eric Lau of the University of Hong Kong, involved 94 patients with COVID-19 admitted to the Eighth People’s Hospital of Guangzhou, China, 50% of whom were male, with a median age of 47 years. Among them, 61 of 93 patients were mild. Specifically, the researchers collected the throat swabs of 94 COVID-19 patients who were treated in the Eighth People’s Hospital of Guangzhou, China from January 21st to February 14th. From the onset of symptoms to 23 days after onset, 414 throat swabs were collected.

The results show that the researchers areThe high viral load was detected shortly after the patient developed symptoms, and then gradually decreased to the detection limit around the 21st day.There is no significant difference in viral load among different sexes, age groups and disease severity.

In addition, the researchers also analyzed 77 samples of "transmission pairs" from public data to assess how much time passed between the onset of symptoms in each patient. It assumes that the median incubation period (the time from infection to symptoms) is 5.2 days.The research team concluded that infectivity began 2.3 days before symptoms appeared, and peaked 0.7 days before symptoms appeared.

In the sensitivity analysis, the same estimation procedure is used, but assuming that the infectious attack is unchanged 1-7 days before the symptom occurs, thenIt was found that the infectivity reached its peak 0-2 days before the onset of symptoms, and the transmission ratio before the onset of symptoms was 46% to 55%.

The study also found that,The virus may start shedding 2-3 days before the first symptoms appear.After the symptoms appear, the viral load in the patient’s body decreases faster. This is consistent with the results of two recent studies. On April 1st, teams from Munich, Germany, Berlin and Cambridge, England published the article "Viro logical assessment of hospital patients with Covid-2019" on Nature. It was found that the shedding peak of Covid-19 occurred in the early upper respiratory tract infection.This shows that virus carriers may be the most infectious when they have mild symptoms or almost no obvious symptoms.

Brother Hong:From the above two documents, we can draw a conclusion that the virus shed and spread 2-3 days before the symptoms appear after infection, and after the symptoms appear, with the passage of time,The viral load of throat swab decreases gradually, and the viral load detected within 7 days after symptoms. If symptoms have existed for more than 20 days, the reliability of negative conclusion of throat swab is poor.

Solution:We need to publicize the law of the new crown to the people and medical workers, and improve our vigilance against COVID-19. It is best for patients to see a doctor in time, and doctors can also suspect this possibility. Only at the right time can we collect high-content samples and diagnose and control them in time.

Second, accurate sampling strategy is the best means to reduce false negative results.

In February, a study published by Medrxir, a pre-printed website, collected respiratory tract samples such as nasal swab, throat swab, sputum and bronchoalveolar lavage fluid (BALF) from patients diagnosed with COVID-19, and included 213 Covid-19-infected patients admitted to the Third People’s Hospital of Shenzhen from January 11th to February 3rd, 2020, who were diagnosed by Guangdong CDC. A total of 866 respiratory tract samples, including 490 nasal swabs, 205 throat swabs, 142 sputum samples and 29 BALF samples, were collected at the time of admission and after each time point. According to the collection date, the samples were divided into 0~7 days, 8~14 days and ≥15 days after onset. According to the guidelines on prevention and treatment of Covid-19 infection issued by National Health Commission, patients were divided into severe and mild cases.

Three groups were divided according to the sample collection time after onset, and the viral nucleic acid was quantitatively determined.The positive rate of viral nucleic acid in sputum samples was the highest in the group of 0~7 days after onset.The positive rates of severe and mild cases were 88.9% and 82.2% respectively.Followed by a nasal swab.(73.3% and 72.1% respectively),The throat swab again.(60.0% and 61.3% respectively). In mild cases, the positive rate of nasal swab and sputum samples is similar.

The positive rate of BLAF samples collected from 8 to 14 days after the onset of severe cases was 100%.However, the BALF samples of 3 mild cases were negative. In all cases, the positive rate of sputum samples was the highest in the 8~14 days after onset, which was much higher than that of nasal swab and throat swab. It is worth noting that the positive rate of throat swab in severe patients is only 50%, and that in mild patients is only 29.6%.

In the group ≥15 days after onset,The positive rates of BALF, sputum and nasal swab from severe cases were similar. In mild cases, the positive rate of sputum and nasal swab is similar, which is much higher than that of throat swab.

For the diagnosis of viral nucleic acid in COVID-19’s patients,Sputum samples are the most accurate, followed by nasal swabs. It is not recommended to collect throat swabs for diagnosis.

The difference between oropharyngeal swab and nasopharyngeal swab

anusswab

Brother Hong:

1. Oropharyngeal swab is the least painful and most acceptable, but it has to bear the consequences of high false negative results. My suggestion is to use it according to the epidemic risk in the first epidemic area.

2. The positive rate of nasopharyngeal swab is higher than that of oropharyngeal swab, which is why nasopharyngeal swab is introduced into laboratory examination in the fifth edition of Diagnosis and Treatment Plan for Pneumonia Infected by Covid-19 (the fourth edition is still pharyngeal swab). If we want to pursue accuracy, it is suggested to use it, or to use it when screening people in close contact or sub-close contact. At present, the examination of returnees in domestic reports seems to be nasopharyngeal swab method.

3. Alveolar lavage fluid can’t be used for screening in the general population, so it is suggested that hospitals with ICU should use it for screening severe pneumonia patients living in ICU for COVID-19 patients.

4. Deep expectoration seems to be the best sample, but many patients in COVID-19 have a dry cough, which may not be possible.

5、anusSwabs were used in Daxing epidemic in Beijing, which is the first time to use this method in the screening process. In some literatures,anusAlthough the positive rate of swabs is not high, as I remember, it seems to be more than 10%, but it does not seem to coincide with the patients with oropharyngeal swabs, suggesting that the virus may attack the gastrointestinal tract, and also suggesting that there may be the possibility of gastrointestinal transmission in Covid-19. Although the initial symptoms of some patients are gastrointestinal symptoms such as abdominal pain and diarrhea, there are also ACE2 receptors in the digestive tract, which is theoretically possible, but it has not been confirmed at present. So,anusMy suggestion is that swabs can only be used as a supplement to nasopharyngeal swabs, and it is better to select patients whose first symptoms are digestive tract symptoms more accurately, which is unlikely to be used as a means of large-scale screening.

Solution:It is best for National Health Commission to issue a guiding document for large-scale screening, and make detailed regulations on what population and what sampling methods to adopt under what circumstances.,When local governments encounter an outbreak in COVID-19, it’s best to ask professionals to act as consultants and make choices according to the actual situation.

Third, the consistency of sampling ability of sampling personnel affects the sampling results.

Garbage in, garbage out (garbage samples produce garbage results), which is a sentence that inspectors often say.

The sampling team is huge, and the ability of sampling personnel must be uneven. In addition, people may have such fears about sampling, which often leads to unqualified samples.

Generally, there is an internal standard in the molecular diagnostic reagent to monitor the detection process, for example, RNaseP is used as the internal standard. If both the sample response curve and the internal standard response curve displayed by the fluorescence PCR platform do not jump, it means that the quality of the collected samples may have problems or false negatives may occur due to improper experimental operation, which needs to attract the attention of medical workers. The RNaseP internal standard can monitor the sample collection and ensure the accuracy of the test results.

Note: RNaseP is ribonuclease P, which is one of the most important ribozymes in human body. It is ubiquitous in all organs, tissues and cells of human body, and can detect RNA nucleic acid fragments from human body samples, and can also be used as an internal standard.

And I privately asked the relevant person in charge of our city’s temporary inspection center. He told me that every day in our hospital, some samples were told that they needed to be re-sampled because the internal standard did not jump. Imagine that you didn’t wipe down a few cells in your throat swab collection, so will the negative of this nucleic acid be accurate?

Solution:Consistency training for nucleic acid sampling personnel will greatly reduce the human influence, and it is easier to standardize the sampling process by taking such sampling methods as nasopharyngeal swab. Our city seems to have trained the nucleic acid emergency sampling team very early. What about your city?

In addition, if in the environment of large-scale nucleic acid sampling, when testing under the standard of 5-mixed 1 and 10-mixed 1, according to the above principle of internal standard, it seems that there is no single-tube ability in the quality control of internal standard. Therefore, especially those in the emergency sampling team, we must pay attention to the standard sampling methods and remember to collect qualified samples as much as possible.

Fourth, choose the right oneSampling device

The virus preservation tube mainly consists of a sampling swab and a virus nucleic acid preservation tube (containing virus nucleic acid preservation solution). There are many kinds of virus preservation tubes on the market at present.It is very important for epidemic prevention and disease diagnosis to choose the appropriate virus preservation tube, reduce the infection risk of medical inspectors as much as possible, and keep the virus RNA stable without degradation..

The preservation solution UTM used in traditional virus collection tubes is based on Hank’s solution, with gentamicin, fungal antibiotics, BSA (V), cryoprotectant, biological buffer and amino acids added. Its main purpose is to maintain the virus activity within a certain temperature range, so that it is convenient for health and epidemic prevention departments to further isolate, culture and trace the virus and determine the pathogen. This kind of non-inactivated tube, which I call it, is good for further work, but the sameLive virus increases the infection risk of medical staff in the whole process of sample collection, transportation and testing!Personally, I think this kind of sampling tube for inactivated virus should be a better choice for disease control sampling.

And clinicalIn order to achieve the purpose of rapid detection, it is not necessary to cultivate the virus, but only to crack the virus, so as long as the integrity and stability of the virus nucleic acid can be guaranteed.nowCommonly known as inactivation tube.Many inactivation tubes in China are used.The inactivation component is guanidine salt, which is in this inactivation tube in our hospital.Guanidine salt can crack the virus, but it can’t protect the integrity of nucleic acid for a long time.The virus nucleic acid may have been degraded during the delivery of the sample to the laboratory, which will also cause a "false negative" in the detection. Many studies show that both temperature and time can affect the test results, so the COVID-19 Laboratory Testing Guide has clear requirements for samples to be tested within 24 hours after collection and transported at 4 degrees Celsius. If it is necessary to exceed 24 hours, it should be stored at -70 degrees Celsius.

And some manufacturers put forward another inactivation tube, a kind of.containNon-guanidine saltThe virus preservation tube containing protein denaturant can inactivate the virus and ensure the preservation of virus nucleic acid at room temperature.And the virus nucleic acid is stably stored for 7 days.Does not degrade.

Solution:

1, from sampling to testing, it is necessary to mark the time, so that quality control can be carried out on the preservation time, which is basically no problem in a better hospital.

2. For the front-line staff to do the corresponding training, they should know the advantages and disadvantages of various types of preservation tubes and adopt the best sampling tubes suitable for local conditions.

3. It is best for the country to recommend the selection of sampling tubes with the support of relevant experimental data.

5. Failure of reagents caused by virus mutation?

After the virus mutation in South Africa, Britain, Brazil and other places, there have been cases that turned positive after ten tests in various parts of the world, including China.The FDA recently warned that virus mutation has caused some reagents to fail, and listed several kits that may be affected by mutation. This means that virus mutation may have led to the failure of some nucleic acid detection or persistent false negative.

The FDA listed several kits that may be affected by mutation:

Accula SARS-Cov-2 detection (Mesa Biotech): If the virus mutates at the 28881st base (GGG to AAC), the detection of this kit will be affected;

TaqPath COVID-19 Combo Kit (Thermo Fisher Scientific): This kit detects three genes at the same time, and the variant B.1.1.7 appearing in Britain will affect one of the detection results;

Linea COVID-19 detection (Applied DNA Sciences): This kit detects two genes at the same time, and the variant B.1.1.7 appearing in Britain will affect one of the detection results;

Helix, an American company doing nucleic acid detection, also noticed the influence of virus mutation on detection. The company’s nucleic acid detection simultaneously detected three Covid-19 genes: ORF1ab, N, and S. Because the B.1.1.7 strain that appeared in Britain had deletion mutation in S gene, ORF1ab and N could be detected in the detection, but S gene could not be detected (4). This phenomenon is called "S gene withdrawal".

In addition, the sensitivity of reagents from different manufacturers to COVID-19 is different, and the country also organized a national evaluation of reagents last year. butIn an evaluation study on the consistency and detection ability of seven domestic novel coronavirus nucleic acid detection kits published by Chinese Journal of Laboratory Medicine in July, 2020, it was proposed that "some kits have poor detection ability for weakly positive samples,It is suggested that weak positive samples should be rechecked by kits from at least two manufacturers to ensure the accuracy of the results."

Several targets of nucleic acid detection in Covid-19 are ORF1ab gene, N gene and S gene.

In the Technical Guide for Laboratory Detection of Pneumonia Infected in novel coronavirus issued by China CDC (hereinafter referred to as the Technical Guide), the two targets of nucleic acid detection in Covid-19 are ORF1ab gene and N gene respectively.

On January 6th, the Weekly Report of China Center for Disease Control and Prevention published the results of gene sequencing analysis of two cases of Covid-19 infection in Shijiazhuang and Xingtai. According to the published results, the strain prevalent in Gaocheng, Hebei Province, is different from the strain found in other places in China recently, and is homologous to the strain reported in Russia last July, belonging to strain B.1.1.123

Coincidentally, the mutation happened at the 28881 ~ 28883 bases of the virus gene of this strain, and this is exactly the position of the forward primer of the N gene!

This also explains why there were many negative results at that time.

I guess this is one of the reasons why the criteria for judging nucleic acid positivity have also changed.The judgment position that the single target is still positive after retest is positive.

Figure/Current Criteria for Judging Positive Cases in Technical Guide

Solution:

1. Require manufacturers of nucleic acid reagents to conduct confirmatory experiments on the current mutant strains and propose technical solutions.

2, all localities should introduce at least two kinds of better quality detection reagents, for control use.

3. Information about the failure of test reagents that may be caused by mutated viruses should be effectively and widely reported in domestic laboratories.

Six, the standard implementation in the process of laboratory testing

the pastAccording to the guidelines issued by relevant institutions, the collected samples need to be inactivated at 56℃ before nucleic acid detection, which is very likely to degrade the Covid-19 nucleic acid, which will lead to the failure of normal detection and ultimately improve the false negative rate.In the early daysInactivation treatment is carried out for the sake of biological safety, to protect the staff engaged in testing from virus infection.

But later, there were many voices of doubt in the academic circles, becauseViral RNA is easily degraded by ribonuclease, because this enzyme has the highest activity at 60℃. Ribonuclease comes from two aspects, one is the sample cells, and the other is the foreign pollutants in the process of collection, preservation and transportation.

Last February, there was a similar research article.The high-temperature inactivation process before virus nucleic acid extraction significantly reduces the amount of detectable virus nucleic acid templates, which has been pre-published on the platform of scientific papers of China Academy of Sciences.

And last May,The Journal of Clinical Chemistry published the research of Chinese scholars on Covid-19’s false negative test.The study confirmed that,Thermal inactivation can cause the increase of Ct value reflected by PCR and cause false negative results.Specifically, 46.7%(7/15) of the weakly positive samples were false negative after inactivation (a surprising proportion). Another inactivation method is to inactivate the virus by dissolving the virus protein with guanidine hydrochloride. This study confirmed that guanidine hydrochloride had little effect on PCR reaction, and the false negative rate was low, although there was also a false negative rate of 13.3%(2/15).

Related address: https://academic.oup.com/clinchem/article/66/6/794/5815979

I also inquired whether there was a 56-degree inactivation step in the detection process of CDC in the United States. Although my English ability was poor, I didn’t see this step by machine translation.

Including some other documents retrieved, it is also very clear that 56-degree inactivation does have a great influence on weakly positive specimens.

No matter the Manual of Nucleic Acid Detection in Medical Institutions in novel coronavirus (Trial) on July 13th, 2020 or the Manual of Nucleic Acid Detection in Medical Institutions in novel coronavirus (Trial 2nd Edition) on December 30th, 2020, there are corresponding descriptions.For the laboratory that has been inactivated with the sampling tube containing guanidine salt, there is no need to inactivate at 56 degrees for 30 minutes.This is only true if non-inactivated sampling tubes are used.

However, I learned from the familiar laboratory leaders that this is not the case. Many laboratories still require samples to be pretreated at 56 degrees for 30 minutes regardless of whether the tubes are inactivated or not. I can understand that they are worried about hospital infection and laboratory exposure. But generally speaking, reducing the false negative rate, increasing the detection amount and taking corresponding measures to control the epidemic situation as soon as possible, I think the corresponding suggestions in the corresponding guidelines are also the best choice from the overall situation and supported by data.

Our laboratory should not control the occurrence of hospital sensation and laboratory exposure at the expense of reducing the detection rate, but should focus on stricter control of experimental steps, more cautious PPE wear and higher laboratory environment.

Solution:

1. It is suggested that the state give special instructions to laboratories at all levels on this matter.

2. It is suggested that the country should further study the preservation solution of sampling tubes, and if there is any preservation solution that can not reduce the detection rate at 56 degrees for 30 minutes, it can also be recommended.

The above zero zero always said a lot of points, mainly about how to reduce false negative conclusions, hoping to give you a reference.

It’s been many days since I wrote this afternoon, mainly considering Spring Festival travel rush. Although the scale of Spring Festival travel rush is reported to have dropped by more than 75% this year compared with previous years, the population flow is still very strong. We may face the rising trend of an epidemic after Spring Festival travel rush, and how to make the work better may be a more urgent need before us.

　　Iranian Foreign Minister Zarif. (Source: Student News Agency)

　　Overseas network September 29 th　Iranian Foreign Minister Zarif said that during his visit to new york, he had a telephone conversation with some members of the US Congress, but unfortunately, he did not hold face-to-face talks with them due to the shortened trip.

　　According to the semi-official Iranian Student News Agency (ISNA) on the 29th, Zarif told reporters when he was preparing to leave new york for Teheran that during his visit, he had talks with different groups in the United States, including some US congressmen. Zarif said that he was called by members of the US Congress. Due to the shortened itinerary, he could not coordinate these members to come to new york for face-to-face talks. He deeply regretted this. Zarif stressed that these US lawmakers have not made any demands on Iran, and Iran will not interfere in the US election. The Iranian side has held talks at the request of the other side and used these talks to show the true image of Iran.

　　Iranian President Rouhani and American President Trump. (overseas network puzzle)

　　When talking about the reports about the meeting between Rouhani and Trump, Zarif said that Trump was only trying to hold talks with Rouhani during his stay in new york this week, and Trump was unwilling to accept the lifting of sanctions as a prerequisite for the talks, so the bilateral talks could not take place. Zarif pointed out that direct talks between Trump and Rouhani have never been put on the agenda, and Iran is only willing to participate in the talks within the framework of the Iranian nuclear deal. Zarif also said that Trump did not promise to lift the sanctions after the talks, but Trump preferred to hold the talks directly and it was possible to lift the sanctions after the talks.

　　The Iranian side’s trip to the United States this time can be described as a thorn all the way. The 74th session of the United Nations General Assembly opened on September 17, 2019. Earlier, Iranian President Rouhani and Foreign Minister Zarif were delayed in obtaining American visas. Rouhani and Zarif "may cancel" their trip "if (the US) doesn’t issue visas within a few hours", Yitong News Agency reported on the 18th. On the same day, Trump said on the Iranian visa issue, "This matter does not depend on me." It was not until 19th that the Iranian delegation to the United Nations announced that the United States issued visas to the Iranian President and Foreign Minister, allowing them to attend the UN General Assembly in new york.

　　Iran said earlier that only when the United States returns to the agreement can the two sides contact. (Photo: Associated Press)

　　On the other hand, the repeated statements of the United States do not rule out the possibility of Trump meeting Rouhani during the UN General Assembly. On the 4th, after imposing additional sanctions on Iran, when asked about the possibility of meeting with Iranian leaders during the UN General Assembly, Trump said that "everything is possible". When he delivered a speech at the UN General Assembly on 24th, Trump also said that the possibility of direct dialogue with Iran was not ruled out, but the sanctions would not be lifted in advance.

　　However, the premise of the Iraqi side is that the United States must lift the sanctions before there can be talks. Iranian Foreign Minister Zarif said on the 22 nd that the premise of holding talks is that Trump promised to ease sanctions against Iran. Iranian President Rouhani also said in his speech at the general debate of the United Nations General Assembly on 25th that Iran has never bowed to foreign forces and refused to negotiate with those who imposed "the harshest sanctions in history". (Compile/Overseas Network Hou Xingchuan)

　　Cctv news(Reporter Li Wenxue): According to the requirements of the "Class B and B Tube" general plan notice of the comprehensive group of the State Council Joint Prevention and Control Mechanism and the "novel coronavirus Infection Prevention and Control Plan (10th Edition)", on January 9th, the Harbin Leading Group Command issued a notice on optimizing and adjusting the nucleic acid detection strategy, requiring that no nucleic acid should be done unless necessary, at least one 24-hour nucleic acid detection point should be set up in all districts and counties, and personnel of key institutions should carry out antigen or nucleic acid detection.

　　According to the notice, it is required to insist on not doing nucleic acid unless necessary, and no longer carry out nucleic acid screening for all staff. Community residents are "willing to check and check" as needed. The cost of nucleic acid testing for personnel and medical personnel in key institutions such as nursing homes and welfare homes shall be borne by the competent department of industry according to the requirements of "who is in charge, who is responsible and who pays"; Other people will pay for nucleic acid testing from January 10, 2023.

　　All districts and counties (cities) should rely on community health service centers or township hospitals to set up enough convenient nucleic acid detection points, and at least one 24-hour nucleic acid detection point should be set up to facilitate the needs of the public. All nucleic acid detection points should be set in place before January 10. All districts and counties (cities) shall set up at least one testing point that can provide Chinese and English nucleic acid testing reports to meet the needs of citizens for nucleic acid testing abroad.

　　Medical institutions should carry out antigen or nucleic acid detection for outpatients (emergency) with fever and respiratory infection symptoms, hospitalized patients with severe and high risk, and symptomatic medical staff.

　　During the epidemic period, the staff in places where vulnerable people are concentrated, such as pension institutions and social welfare institutions, will carry out full-time nucleic acid testing twice a week, and the people who are cared for will carry out antigen or nucleic acid testing twice a week. The testing of agency staff and people who are cared for will be carried out alternately every other day, or it can be carried out according to the requirements of the competent authorities of the industry. Nucleic acid detection of people in other industries shall be carried out in accordance with the requirements of the competent department of industry.

　　The elderly aged 65 and above, long-term hemodialysis patients, severe diabetes patients and other high-risk community residents, infants under 3 years old and other people need to carry out antigen testing after symptoms such as fever and respiratory infection, or go to convenient nucleic acid testing points set up in the community for nucleic acid testing. Just as residents are infected, other people can carry out antigen testing every day for 3 consecutive days.

　　After other residents have symptoms such as fever and respiratory infection, they can carry out antigen testing on their own as needed, or go to convenient nucleic acid testing points set up in the community for nucleic acid testing.

　　Foreigners enter the gathering place of vulnerable people, check the negative certificate of nucleic acid test within 48 hours and carry out antigen test on the spot; Enter all kinds of business premises, commercial stores, government agencies, enterprises and institutions, and no longer check the negative certificate of nucleic acid detection.